|
KMID : 1059519930370030355
|
|
Journal of the Korean Chemical Society
1993 Volume.37 No. 3 p.355 ~ p.363
|
|
|
Chemical Synthesis of Oligodeoxyribonucleotides Using Paper Disk as a Support
|
|
Ro Bong-Oh
Kim Sung-Jun
Shin Dae-Hyun
|
|
|
Abstract
|
|
|
|
The two deoxyribonucleotides, 5’-d(GAATTCCGGCCA) and 5’-d(CGAGCTGTC), were synthesized on disks of Whatmann chromatography paper (3MM) by the phosphite-triester method. The average yield of the dodecamer and the nonamer that was measured by trityl analysis is 76.1% and 86.5% respectively. Separation of the synthesized oligodeoxyribonucleotides were purified by HPLC. The two oligodeoxymers’bases were quantified by HPLC with snake venom phosphodiesterase and bacterial alkaline phosphatase. The numbers of bases were equal to the designed ones. The advantage of using a paper disk as a support allows us to synthesize many different oligodeoxyribenucleotides during one synthesis. This method is the most convenient synthetic technique to synthesize oligodeoxyribonucleotides easily and inexpensively in a relatively short time.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|